Eric R. Hugo
eric at bcserv.WUSTL.EDU
Thu Nov 18 09:57:38 EST 1993
I use a method involving powdered glass for purifying phagemid
ssDNA that might work for PCR ssDNA. Basically:
1. Bind ssDNA to 5 ul of glassmilk in 4M NaClO4 (1 ml final)
2. Pellet glassmilk resuspend in 4M NaClO4 (1 ml)
3. Wash 2-3 X in 50% ethanol:10 mM tris: 1 mM EDTA ph 7.5.
4. Dry pellet.
5. Resuspend in 10 ul of TE heat to 50 C 5-10 min, pellet
ssDNA should be in the sup.
Eric R. Hugo, Postdoctoral Research Associate |eric at bcserv.wustl.edu
Dept. of Biochemistry and Molecular Biophysics|finger above for
Washington University School of Medicine |public key <--
Box 8231, St. Louis, MO 63110_________________| (314) 362-3342
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