Restriction Digests + Microwave??
wfischer at bio.indiana.edu
Fri Nov 19 13:45:07 EST 1993
Peter Kaub (p-kaub at csu.murdoch.edu.au) wrote:
: on using microwave radiation for enhancing restriction endonuclease reactions.
: this is new to me and I would be interested if anybody had any
: information on this technique. Does it increase efficiency of cutting?
: Does it decrease time needed for complete cutting?
See A.K. Jhingan (1992) Microwave Restriction Enzyme Digestion of DNA.
Methods in Molecular and Cellular Biology 3:270-274.
Abstract: A simple technique has been developed for digesting
genomic as well as plasmid DNA utilizing a microwave oven. The
digestions are complete in minutes and are highly reproducible.
Haven't tried it yet. Has anyone else?
wfischer at indiana.edu
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