X-Gluc chromogenic substrate
William_D_WARREN at UMAIL.UMD.EDU
Fri Nov 19 16:52:00 EST 1993
>Can some one enlighten me. What applications is X-Glu use for?
I guess that you are refering to the chromatographic substrate for
beta-glucoronidase which is analogous to X-gal.
What are it's applications ??? Endogenous B-galactosidase activity is very
high in many organism, particularly plants, so X-gal reporter constructs are
pretty useless. B-glucoronidase on the other hand appears appears to be far
less ubiquitous than B-gal. Many people who want the convenience of
X-gal reporter gene detection but can't use it because of high endogenous B-gal
activity use the B-Gluc reporter instead. See Jefferson et al PNAS 83 pages
Also now that there are many different color-substrates available
I guess that you could also monitor both a B-glu reporter as well as a B-gal
reporter simultaneously using different chromophores if it took your fancy.
William D. WARREN, PhD
Center for Agricultural Biotechnology Email: ww40 at umail.umd.edu
University of Maryland at College Park Phone: (301) 405-7681
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