X-Gluc chromogenic substrate

ww40 William_D_WARREN at UMAIL.UMD.EDU
Fri Nov 19 16:52:00 EST 1993


>Hi Netters,
>
>Can some one enlighten me. What applications is X-Glu use for?
>
>Duncan
>-- 

I guess that you are refering to the chromatographic substrate for
beta-glucoronidase which is analogous to X-gal.

What are it's applications ??? Endogenous B-galactosidase activity is very
                                            ^^^^^^^^^^^^^
high in many organism, particularly plants, so X-gal reporter constructs are
pretty useless. B-glucoronidase on the other hand appears appears to be far
less ubiquitous than B-gal.  Many people who want the convenience of
X-gal reporter gene detection but can't use it because of high endogenous B-gal
activity use the B-Gluc reporter instead. See Jefferson et al PNAS 83 pages
8447-8451.

Also now that there are many different color-substrates available
I guess that you could also monitor both a B-glu reporter as well as a B-gal
reporter simultaneously using different chromophores if it took your fancy.

BILL

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William D. WARREN, PhD
Center for Agricultural Biotechnology     Email: ww40 at umail.umd.edu
University of Maryland at College Park    Phone: (301) 405-7681 

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