Electroporation of mosquito cells

Hinayana Bawagan bawagan at umdnj.edu
Mon Nov 22 20:14:20 EST 1993


     I am using the Biorad electroporator.  I have found only one paper on electroporation of insect cells.  This one is on electroporation of baculovirus DNA
into Spodoptera frugiperda cells.  Does anybody have any suggestion on the 
parameters I should try? I suppose insect cells are less hardy than mammalian 
cells.  I have electroporated RNA into BHK21 cells using 850V, 25 uF (pulsed 2X).     All feedback is welcomed and appreciated.



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