Electroporation of mosquito cells
bawagan at umdnj.edu
Mon Nov 22 20:14:20 EST 1993
I am using the Biorad electroporator. I have found only one paper on electroporation of insect cells. This one is on electroporation of baculovirus DNA
into Spodoptera frugiperda cells. Does anybody have any suggestion on the
parameters I should try? I suppose insect cells are less hardy than mammalian
cells. I have electroporated RNA into BHK21 cells using 850V, 25 uF (pulsed 2X). All feedback is welcomed and appreciated.
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