bhandary at wccf.mit.edu
Mon Nov 22 20:13:00 EST 1993
In article <belas.180.000C49E3 at mbimail.umd.edu>, belas at mbimail.umd.edu (Bob Belas) writes...
>I have a couple of questions regarding preparation of ssDNA from pBLUESCRIPT
>II using the M13K07 helper phage. First, can I propagate the helper phage
>using the same protocols I use for regular M13 (adding kan for selection).
>Second, would someone point me to a good technique for using phagemids? I
>currently have Sambrook et al and Ausabel et al in front of me. Are there any
>other sources for methodology that I should look at? Thanks.
I've propagated the helpers in BB4, as well as JM103 (which I use for regular
M13's). The titers are lower (by maybe 100x?) than one gets from M13.
I've used protocols supplied by a Promega Handbook (not their catalog; they
have a separate protocol book that is really quite nice). I'm sure they would
send you a copy if you request it.
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