Cleaning sequencing gel plates
krasel at alf.biochem.mpg.de
Sun Nov 21 14:45:52 EST 1993
Jim Owens (jow at helix.nih.gov) wrote:
: In article <931116174415_76467.702_BHA63-1 at CompuServe.COM> Alan Gerstein,
: 76467.702 at CompuServe.COM writes:
: >Do you use 0.5 - 1.5 M sodium hydroxide to clean your glass plates?
: The only reason to use strongly basic cleaners is to remove the silane
: applied to the plates. For that, we use warm KOH by dissolving KOH in a
: small amount of water and smearing it over the plates for 15 seconds or
In my experience, it is also possible to get rid of silane (repel silane
as well as binding silane) by cleaning the plates with acetone (after
the usual procedures with water/detergent).
Just my $0.02, Cornelius.
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de fax: +49 89 8578 3975 */
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