Why LB or TB?

[Michael Benedik]

In article <leach-221193093509 at med-pharm5.bu.edu>, leach at mbcrr.harvard.edu (Martin Leach) writes:
>Dear netter,
>
>For DH5-alpha bacteria containg any plasmid we routinely grow them in LB
>broth. One colony per 5 ml overnight. Or 100 ul of glycerol stock per 500
>ml overnight.
>I think if you need exceptional yields you should look into using
>cycloheximide. I think this is used to amplify plasmids within bacteria (i
>know on plate) without further division.
>
>Good Luck 
>
>Martin Leach
>
>-- 
>
>......          Martin Leach                Email:leach at mbcrr.harvard.edu 
>   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
>   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
> _/  |-/__==/  80 E. Concord St. (L603)
>(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
>               USA                           head.....WIBBLE" -BLACKADDER  



CHLORAMPHENICOL (or Spectinomycin). NOT cyclohexamide.


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 Michael Benedik				INTERNET: Benedik at uh.edu
 Dept. of Biochemical & Biophysical Sciences	
 University of Houston				BITNET: Benedik at uhou
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