Why LB or TB?
leach at mbcrr.harvard.edu
Mon Nov 22 04:35:09 EST 1993
For DH5-alpha bacteria containg any plasmid we routinely grow them in LB
broth. One colony per 5 ml overnight. Or 100 ul of glycerol stock per 500
I think if you need exceptional yields you should look into using
cycloheximide. I think this is used to amplify plasmids within bacteria (i
know on plate) without further division.
..... Martin Leach Email:leach at mbcrr.harvard.edu
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