PstI and NheI-sites

Paul Riggs riggs at
Tue Nov 23 12:04:49 EST 1993

It shouldn't happen, but if there's some ss nuclease around to blunt the
ends, and the ligase concentration is high enough, the plasmid can reclose
by blunt-end ligation. Using less ligase prevents this.

In article <1993Nov12.183558.1 at>, malaine at
> Hi!
> Is it possible that a plasmid having Pst I- and Nhe I-cohesive ends can
> religate into itself?
> Marko Laine
> -- 

Paul Riggs
New England Biolabs
riggs at

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