PstI and NheI-sites

Paul Riggs riggs at neb.com
Tue Nov 23 12:04:49 EST 1993


Marko
It shouldn't happen, but if there's some ss nuclease around to blunt the
ends, and the ligase concentration is high enough, the plasmid can reclose
by blunt-end ligation. Using less ligase prevents this.
Paul

In article <1993Nov12.183558.1 at sara.cc.utu.fi>, malaine at sara.cc.utu.fi
wrote:
> 
> Hi!
> 
> Is it possible that a plasmid having Pst I- and Nhe I-cohesive ends can
> religate into itself?
> 
> Marko Laine
> -- 

______
Paul Riggs
New England Biolabs
riggs at neb.com



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