Lane broadening on protein gels

David I Resnick dresnick at athena.mit.edu
Mon Nov 22 23:05:48 EST 1993


In article <9311220242.AA03948 at pines2.ljcrf.edu> helgew at LJCRF.EDU (Helge Weissig) writes:

   >Hi gurus,

   [stuff deleted]

   >When the gels runs everything looks great but on staining the bands are 2-3
   >times the width of the lane. The MW standards run fine as do other gels with
   >different samples. Clearly the problem seems to be in the sample
   >preparation. Any hints?????
   >Michael K Holland PhD
   >CSIRO, Wildlife & Ecology
   >PO Box 84, Lyneham ACT 2602, Australia
   >Fax: 61-6-242-9242  Phone:61-6-242-1793
   >mholland at dwe.csiro.au

You can see this effect with too much salt in your sample.  E.g. when
adding 5xsample buffer to sample, if the [Salt] > about 500 mM you may
see this happening.  Of course, there may be some other problems
too...

--
       David Resnick                           dresnick at athena.mit.edu      



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