Xsomal DNA minipreps

Duncan Clark Duncan at genesys.demon.co.uk
Wed Nov 24 14:14:31 EST 1993


Hi Netters,

I need to get some Xsomal DNA from 130 Thermus isolates. 1/8 plates have been
scraped off and resuspended in 200ul 10mM Tris, 10mM EDTA and frozen. Has any
one a simple protocol, preferably not using phenol extractions, that will give
me a few ug of DNA (it is only going to be used for PCR). If I can help it I 
don't want to do multiple extractions/transfers from eppendorf to eppendorf 
etc. The bugs lyse easily. All suggestions welcome.

Many thanks 
-- 
Duncan Clark



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