Do you have an unclonable gene?

[Kay Hofmann]

In article <2be6ea$2uc at gazette.bcm.tmc.edu>, pburch at cmb.bcm.tmc.edu (Paula Burch) says:
>
>A colleague of mine, Tamsen Valoir, is compiling a list of sequences
>that have been difficult or impossible to clone. Her specific interest
>lies in the reasons for said difficulty, e.g., sequences that are 
>toxic to the host cell, sequences that get deleted, methylated 
>sequences, etc.
>
I would love to send you the sequences of two or three very nasty
genomic fragments but sorry, we encountered some difficulties in 
cloning them... :-)

I beg your pardon,
                                 Kay