Coupled vs uncoupled in vitro transcription/translation

Jim Woodgett jwoodget at
Thu Nov 25 17:10:31 EST 1993

Has anyone had good/bad experiences with Promega's TnT coupled 
transcription/translation system?  We've always made RNA first then 
translated in retic. lysates but this does take more time than the coupled 
methods.  Any comparisons between the two apart from cost?

Jim Woodgett      Fax (416) 926 6529     Tel (416) 924 0671
jwoodget at

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