Quantify RNA transcripts
Hinayana Bawagan
bawagan at umdnj.edu
Mon Nov 8 20:17:36 EST 1993
I use RNA transcripts directly from the transcription reaction (w/o DNAse
treatment) for electroporation. I run TBE 0.5% agarose gel to check tthe
quality of my RNA transcripts using HindIII lambda DNA markers. I get a nice
thick band but I have not yet quantified my RNA. I know the concentration of mystarting DNA template and according to the company protocol I am following that starting with 1.5-2 ug of DNA I will end up with up to 12- 25 ug. But I want tobe able to obtain the actual value not just based on prediction.
Thank you very much/
More information about the Methods
mailing list