Yeast X-gal plates

Dr. S. Bhattacharya sbhattac at crc.ac.uk
Mon Nov 29 04:24:08 EST 1993


Hi netters, could someone tell me how to avoid getting a turbid 
precipitate when I make yeast X-gal plates.  I use the recipe in 
current protocols in Mol Biol, autoclave the premix and dropout 
powder, cool to 50deg C, add the 0.7 M potassium phosphate pH7, and
bingo, a precipitate appears.  The plates seem to work OK, so is this
a normal phenomenon?
Thanks
Shoumo
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Shoumo Bhattacharya		
MRC Molecular Medicine Group
Royal Postgraduate Medical School
Hammersmith Hospital, LondonW12 0NN UK
Tel:081-7435566 ext.2343
Fax:081-7498341
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