Skew sequences

Ashleigh Mckenzie MCKENZIEA at gate2.cc.unp.ac.za
Mon Nov 29 08:58:28 EST 1993


To those who know anything about sequencing:

Can anyone suggest ways to stop sequenced DNA lanes running skew
in the gel? We use a poker face II apparatus and prerun for 2 hrs
at 50 - 60 W, followed by sample run at 60W. The temperature
gradient across the gel is not even, being much hotter on the
edges and near the bottom of the plates. Changing the wattage at
which the gel runs has not helped much.

Thanks in advance

Ashleigh Mckenzie

mckenziea at botany.unp.ac.za



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