Source of lac repressor??
bhandary at wccf.mit.edu
Tue Nov 30 16:35:00 EST 1993
In article <ibelgaufts-241193153435 at lmubmac25.biochem.mpg.de>, ibelgaufts at vms.biochem.mpg.de (horst ibelgaufts) writes...
>does anyone know of a source of lac repressor?
>Institute of Biochemistry, c/o MPI of Biochemistry
>Munich Martinsried, FRG
For what it's worth, STRATAGEN now sells a two vector system having the lac
repressor on one vector, and a lac-operator/multicloning site system on the
other. The repressor in question has been modified to have a nuclear
targeting system for mammalian cells, yet still retains excellant affinity for
the lac operator. If you get the repressor vector, you could use it as a
starting point (subclone into an E.coli expression system on a puc based
vector) for large scale production. The lacIq is present on many of the
f-factors in M13 strains, but I don't think you could get enough that way.
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