Lambda-exo to make sDNA

Johan de Boer jdboer at SOL.UVIC.CA
Mon Nov 29 14:58:55 EST 1993


Hello,

A while back there was some talk I believe about using lambda exonuclease
to turn a double stranded PCR fragment into a single strand. The PCR was 
done with one of the primers kinased. Has anyone used this method, was 
there a reference to it, does anyone have comments on its use?

Thanks, 

Johan de Boer (jdboer at sol.Uvic.CA)
Centre for Environmental Health
Victoria, Canada



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