Recovery of DNA from acrylamide gels
ds1539 at u.cc.utah.edu
Fri Nov 26 22:31:37 EST 1993
Duncan Clark (Duncan at genesys.demon.co.uk) wrote:
: Hi Netters,
: Yet more questions!
: Does anyone have an efficient method of recovering DNA fragments from
: acryalmide gels, size of the order of 4.5kb?
: As an aside. Agarase digests agarose. Does anyone know of an enzyme that will
: digest acrylamide?
Bio 101, the makers of GENECLEAN, sell a kit called MERMAID which is
marketed to extract oligo-MERs out of acrylamide gels. I don't know
how well it works on big fragments, but you might call them and see
what they recommend. Actually, I'm surprised they you are purifying a
4.5 kb frag from acrylamide; isn't that kind of large? Couldn't you use
- Good Luck!
- Dave Segal
- Biochemistry, U. of Utah
- Dave.Segal at m.cc.utah.edu
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