Extracting DNA from bone

APARKER at MAINE.MAINE.EDU APARKER at MAINE.MAINE.EDU
Fri Nov 26 12:20:35 EST 1993


In article <2d1cbmINN46p at zephyr.grace.cri.nz>, srgcles at grv.grace.cri.nz says:
>
>Extracting amplifiable DNA from bone
>
>I am working on mtDNA amplification and sequencing for identification of human
>remains. My primers target hypervariable regions I & II in the mtDNA D-loop. I
>presently have three sets of bones, including one skull with dentition(no
>dental work), from
>unidentified individuals which have been found in new Zealand. The skull has
>probably been underwater for 34 years.
>
> We have
>possible maternal relatives for two of the sets of bones, and both are keen to
>provide reference samples.
>
>My problem is a protocol for extracting amplifiable DNA from bone. Has anyone
>reading this done this ? I have not yet attempted extraction from these bones
>and would like some guidance. In particular:
>1) Are some bones (ie teeth) better for extraction than others? What are the
>best targets likely to be?
>2) Can anyone point me to a failsafe (if there is such a thing) protocol for
>the extraction
>3) Is any specialised equipment required?

Check out NAR v.21 n.16 pp.3913-3914 for an article on DNA from pleistocene
horse bones via binding to glassmilk in a guanidine thio-cyanate buffer.

I wish there was a foolproof technique for such sketchy samples, but there
ain't.  Good luck.
Alex Parker



More information about the Methods mailing list