Steven Russell Genetics
sr120 at mbfs.bio.cam.ac.uk
Fri Nov 26 12:13:09 EST 1993
> In article <9311251954.AA09492 at fraser.sfu.ca> , suisheng at sfu.ca writes:
> > I have a question about a cDNA library screen. I don't know why there
> > are a few big round(2-4 mm diameter) clear plaques among those normal
> > size plaques in the plates.
If the library is really valuable it is possible to try and streak out E.coli
from the middle of the offending plaques and thus isolate a strain resistant
to the contaminating phage. Then titre your library on several different
single colony isolates and use the one which gives you the highest titre. I
have done this and it seems to be ok.
Dept of Genetics
University of Cambridge
SR120 at mbfs.bio.cam.ac.uk
More information about the Methods