Extracting DNA from bone

[scarr at kean.ucs.mun.ca]

In article <2d1cbmINN46p at zephyr.grace.cri.nz>, srgcles at grv.grace.cri.nz writes:
> Extracting amplifiable DNA from bone
> 
> I am working on mtDNA amplification and sequencing for identification of human 
> remains. My primers target hypervariable regions I & II in the mtDNA D-loop. I 
> presently have three sets of bones, including one skull with dentition(no 
> dental work), from 
> unidentified individuals which have been found in new Zealand. The skull has 
> probably been underwater for 34 years.
> 
>  We have 
> possible maternal relatives for two of the sets of bones, and both are keen to 
> provide reference samples.
> 
> My problem is a protocol for extracting amplifiable DNA from bone. Has anyone 
> reading this done this ? I have not yet attempted extraction from these bones 
> and would like some guidance. In particular:
> 1) Are some bones (ie teeth) better for extraction than others? What are the 
> best targets likely to be?
> 2) Can anyone point me to a failsafe (if there is such a thing) protocol for 
> the extraction
> 3) Is any specialised equipment required?
> 
> I appreciate any assistance which can be given
> 
> Brian Scrimshaw
> Environmental Science and research, Forensic
> Wellington
> New Zealand

Brian -

M. Hoss and S. Paabo (1993). DNA extraction from Pleistocene bones by a
silica-based purification method. "Nucleic Acids Research" 21(6):3913-4.

This protocol is extremely detailed and works well for us.

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Steven M. Carr
Dept. of Biology
Memorial University of Newfoundland
St. John's NF A1B 3X9
CANADA

(709) 737-4776 office / -4713 lab / -4000 FAX
scarr at kean.ucs.mun.ca
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