Problems with inclusion bodies

Karina Landl Landl at bkfug.kfunigraz.ac.at
Fri Oct 1 11:38:15 EST 1993


Hey folks out there!
My enzyme (squalene epoxidase from yeast) expressed in E.coli is
enriched in inclusion bodies. In order to maintain the enzyme4s activity
I cannot use triton X-100 or Urea to solubilize them. Who knows
something about alternatives ? 
All suggestions are very welcome !
Karina



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