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beta galactosidase in cultured cells

Martin Kennedy mkennedy at chmeds.ac.nz
Tue Oct 5 21:23:18 EST 1993

In article <leach-051093092638 at med-pharm5.bu.edu>, leach at mbcrr.harvard.edu (Martin Leach) writes:
> In article <andres.37.749754284 at calvin.jci.tju.edu>,
> andres at calvin.jci.tju.edu (Andres Ferber) wrote:
>> Does anybody know of a reference for staining mammalian cells transfected
>> with betagalactosidase constructs?
>> I am interested in staining the cells alive by adding Xgal to the culture
>> media without fixing the cells.  Is it possible? Any suggestions or comments?
>> Andres Ferber

> I have an old protocol that works well, instead of posting phone PROMEGA at
> 1-800-356-9526 and they can fax you their protocol in their b-gal assay
> booklet.
> Martin Leach


I've done X-gal staining on fixed mamalian cells expressing lacZ, but can you
really do it on live cells without fixing????


NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
		Phone (64-3)364-0880  Fax (64-3)364-750

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