beta galactosidase in cultured cells
mkennedy at chmeds.ac.nz
Tue Oct 5 21:23:18 EST 1993
In article <leach-051093092638 at med-pharm5.bu.edu>, leach at mbcrr.harvard.edu (Martin Leach) writes:
> In article <andres.37.749754284 at calvin.jci.tju.edu>,
> andres at calvin.jci.tju.edu (Andres Ferber) wrote:
>> Does anybody know of a reference for staining mammalian cells transfected
>> with betagalactosidase constructs?
>> I am interested in staining the cells alive by adding Xgal to the culture
>> media without fixing the cells. Is it possible? Any suggestions or comments?
>> Andres Ferber
> I have an old protocol that works well, instead of posting phone PROMEGA at
> 1-800-356-9526 and they can fax you their protocol in their b-gal assay
> Martin Leach
I've done X-gal staining on fixed mamalian cells expressing lacZ, but can you
really do it on live cells without fixing????
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-750
More information about the Methods