OmpA expression vectors--request
bsh at MED.PITT.EDU
Wed Oct 6 13:45:20 EST 1993
> Dear Bionetters,
> I recently discovered that my stab of a strain containing
> the plasmid pINIII ompA1 was dead. I'm trying to track down
> a source of this expression vector (E. coli). If anyone
> out there has a stab or a small quantity of plasmid DNA they
> could and would spare I would be highly appreciative. Also
> if anyone else has a noncommercial expression/secretion vector
> for E.coli I would be interested.
> Many thanks!
> Eric R. Hugo, Postdoctoral Research Associate |eric at bcserv.wustl.edu
> Dept. of Biochemistry and Molecular Biophysics|finger above for
> Washington University School of Medicine |public key <--
> Box 8231, St. Louis, MO 63110_________________| (314) 362-3342
There was an article about a year or so back in BioTechniques which described
a solution for the exact problem you have. I don't remember the exact
reference but the gist was that, take the stab or the culture (dead) and
do a DNA extraction. although you may not see any DNA, specially the plasmid,
there will be enough to support a transformation and get your clone back.
I think it is worth a try.
bsh at med.pitt.edu
Univ of Pittsburgh
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