my experience with Biorad posphoimager GS250
jgraham at bronze.ucs.indiana.edu
Thu Oct 7 10:07:50 EST 1993
We have been using the Molecular Dynamics Phosphoimager here in the
Biochemistry section of the Chemistry Department for about two years now
without any significant technical problems. Runs on a 486 Pee-Cee. The
company claims six order of magnitude accuracy in quantitation. It also
requires spotting of known label to give cpm, and does not indicate the
length of exposure. Sensitivity is simillar to autoradiography, in that
the image looks simillar, with larger background usually from the imager.
The only "sensitivity" difference is that hot gels can be exposed for
short periods (1-2 h) and give similar results to overnight autorads, and
you can adjust the pixel representation, in order to "bring out" weak
bands. This can sometimes allow reading of DNA sequence information that
is too unclear to be seen in autoradiography.
Because of the accuracy of quantitation and the reduced expense of film
images, I consider phosphimaging a major advance in standard practices.
Indiana University Bloomington
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