Screening for Protein-Protein Interactions

GARCIA02 at swmed.edu GARCIA02 at swmed.edu
Thu Oct 7 15:39:02 EST 1993


we are currently screening some expression libraries looking for 
protein-protein
interactions using a GST-Fusion protein.  In our initial controls GST 
by itself
gives no background, bu

t when we use the GST-fusion protein every 
plaque on the
filter is lighting (suggesting that our protein is interacting with 
either a col
i or phage protein).  We've tried different hybridization conditions, 
detergents
, blocking procedures among others, but are running out of ideas.  We 
would appr
eciate any suggestions.



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