polyanion heparin

Dr. C.P. Healy chealy at crc.ac.uk
Fri Oct 8 11:16:31 EST 1993

In article <1993Sep28.143115.4377 at rockyd.rockefeller.edu> ralston at rockyj.rockefeller.edu (Diana Ralston) writes:
>Heparin is a mucopolysaccharide sulfuric acid ester that
>prolongs the clotting time of blood.  It is highly negatively
>charged (hence polyanionic) and as such somewhat chemically
>mimics nucleic acid.  We used it in my old lab as a competitor
>of protein-DNA interactions; it can "soak-up" up DNA-binding
>proteins, for example, RNA polymerase or transcription factors
>that are attracted to all that negative charge.  I haven't
>heard it used as an RNAse inhibitor before, but I suppose the
>same principle applies.  I can't recall where we purchased it,
>but I have a vague memory of it being in a Sigma bottle.  It
>should be generally available . . . be sure to look for it
>just as Heparin.  Heparin sulfate is also an affinity matrix
>for purifying DNA-binding proteins.
>Diana Horvath
>ralston at rockvax.rockefeller.edu
Isn't "HEPARIN" an acronym for HEPAtic Rnase INhibitor?

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