GC-rich sequencing problem

Dr. Frank A. Simmen's Lab fas-lab at animal.ufl.edu
Fri Oct 8 14:05:49 EST 1993


Hello netters,

I am sequencing a GC rich genomic fragment. The vector in which it is 
subcloned is pGem3Z.  I use the Sequenase 2.0 kit from UNSB. The problem is 
that I always get compression. Does anybody have any ideas or suggestions 
how to solve this problem.

Thanks in advance,

Sihong Song.



More information about the Methods mailing list