lambda gt10 wanted

Martin Kennedy mkennedy at chmeds.ac.nz
Thu Oct 14 20:16:45 EST 1993


In article <29huo1$koa at noc.usfca.edu>, Chihara at usfca.edu (Dr.Chihara) writes:
> I have a student who would like to grow up a lot of vector phage
> without foreign inserts to use as a standard DNA.  Does she have to
> package the DNA which I have found sources for, or is someone out there
> willing to share some phage?   

You can introduce lambda DNA (and of course M13, RF or ss) into E.coli with a
low efficiencey by normal transformation procedures;  I seem to recall this
works at about 10^3 plaques/ug, depending on the strain etc.  This might save
you bothering to package it.

Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
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