lambda gt10 wanted
Martin Kennedy
mkennedy at chmeds.ac.nz
Thu Oct 14 20:16:45 EST 1993
In article <29huo1$koa at noc.usfca.edu>, Chihara at usfca.edu (Dr.Chihara) writes:
> I have a student who would like to grow up a lot of vector phage
> without foreign inserts to use as a standard DNA. Does she have to
> package the DNA which I have found sources for, or is someone out there
> willing to share some phage?
You can introduce lambda DNA (and of course M13, RF or ss) into E.coli with a
low efficiencey by normal transformation procedures; I seem to recall this
works at about 10^3 plaques/ug, depending on the strain etc. This might save
you bothering to package it.
Cheers,
Martin
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-0750
More information about the Methods
mailing list