Substrate Assays

P. Knygsman fred at gpu.utcc.utoronto.ca
Fri Oct 15 15:16:12 EST 1993


Are substrate assays your forte? Here is one for you.

I want to use an Fmoc...~Na (heptapeptide) substrate in a liquid
enzyme assay.  I want to make a stock solution of 1.0mM (or
greater) and use it at a final concentration of 0.8mM in the assay. 
I have tried to solubilize it in 20mM and 50mM TRIS, at pH 6.0, 6.5
and 7.0.  I have tried the same with 50mM KH2PO4.  The results
showed higher solubility in both buffers at pH 6.5.  I have also
tried using DMSO up to a final concentration of 8%, this does not
solubilize it either.  If you have any input, it would be greatly



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