CAT assay

norm eberhardt eberhard at Mayo.EDU
Mon Oct 11 07:38:09 EST 1993


In article <9310092014.AA25153 at cardinal.ncsc.org>, mbwhite at CARDINAL.NCSC.ORG (Martyn White) writes:
|> 
|> 
|> 	Some time ago, someone posted a method for a quick and
|> easy CAT assay involving [3H]chloramphenicol.  The acetylated
|> chloramphenicol is separated by solvent extraction and measured
|> by scintillation counting.  If anyone has saved this method
|> or has a reference, please could you email or post.
|> 
|> Thank you
|> 
|> Martyn White
|> 

	The two phase diffusion assay was initially published by Neudmann et al.
and subsequently modified by Eastman.  References are:

Neumann, J.R., Morency, C.A. and Russian, K.O. (1987). 
Biotechniques 5, 444447.

Eastman, A. (1987).  Biotechniques 5, 731732.

Norman Eberhardt
Mayo Clinic
Rochester, MN



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