pUC118/9 sequence(try pUC18c)

dallas at mcvax4.d48.lilly.com dallas at mcvax4.d48.lilly.com
Mon Oct 11 10:10:23 EST 1993


In article <1993Oct8.070231.9164 at abo.fi>, JANIEMI at FINABO.ABO.FI (Jarmo Niemi) writes:
> In <292qg8$9k0 at menudo.uh.edu> bchs1b at Rosie.UH.EDU writes:
> 
>> In article <1993Oct6.202049.1 at molbiol.ox.ac.uk>, adavies at molbiol.ox.ac.uk (Anthony Davies) writes:
>> >Can anyone direct me to the sequence of pUC118 and 119?  18 and 19 are 
>> >everywhere, but these derivatives I cannot find - even at Welchlab!
> 
>> I got it some time back from the databases which came with the GCG 
>> programs. Either it was in Genbank or in the Vector database. If you
>> can not find it, let me know and I can send it to you.
> 
>>  Michael Benedik				INTERNET: Benedik at uh.edu
> 
> Huh ? Couldn't find it in our GCG (Genbank 78, EMBL 35):

It may be known as pUC18c.  Several years ago I built a pUC118 and pUC119 from
the Messing publication (Methods in Enzymology).  Send me a e-mail message and
I will send back the sequence.

E-mail  internet  jrm at lilly.com

> 
> ! STRINGSEARCH from: GenEMBL:*  October 8, 1993  08:52
> 
> ! searching for: "puc118"           ..
> 
> ! Sequences searched: 135824
> 
> ! STRINGSEARCH from: vec:*  October 8, 1993  08:54
> 
> ! searching for: "puc118"           ..
> 
> ! Sequences searched: 140
> 
> Could you tell me where it is, or maybe even post the sequence ? I have
> noted many people looking for it !
> 
> Jarmo Niemi   Biochemistry, University of Turku, Finland, janiemi at finabo.abo.fi
> 
Jim Miller
Lilly Research Labs
Indianapolis, IN



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