Ligation

ramacha at CC.UManitoba.CA ramacha at CC.UManitoba.CA
Tue Oct 12 12:26:46 EST 1993


> > Greetings Netters,
> > 
> > I am having an tough time trying to run a ligation reaction.
> > 
> > I am trying to ligate a 4.1 kb Hind III Sal I fragment into a Hind III Sal
> > I interval in the multicloning site of a YIp 5 derivative plasmid (3.9 kb).
> > 
> > I use BRL (5u/micro l) T4 DNA ligase and I also Alk. Phos. the vector.
> > I also tried blunt end ligation.
> > 
> > Have any of you had similar problems .
> > 
> > I would greatly appreciate any suggestions/ comments.
> > 
> > I will mail a summary of the suggestions I recieve.
> > 
> > Thanx very much for your help.
> > 
> > Karthikeyan R.
> > 
> > ---------------------------------------------------------------------------
> > Karthikeyan Ramachandran [Karthi]
> > Dep. of Microbiology,                      Ph. Res. (204) 275-7812
> > Univ. of Manitoba,                         Ph. Off. (204) 474-9227
> > Winnipeg, Manitoba,                        Fax. (204) 275-7615
> > Canada R3T 2N2                             e-mail ramacha at ccu.umanitoba.ca
> > 
> > 
> > 
> 
> 



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