Dean Danner Lab
djdlab at sun.com
Tue Oct 12 15:57:59 EST 1993
In article AA25153 at cardinal.ncsc.org, mbwhite at CARDINAL.NCSC.ORG (Martyn White) writes:
> Some time ago, someone posted a method for a quick and
>easy CAT assay involving [3H]chloramphenicol. The acetylated
>chloramphenicol is separated by solvent extraction and measured
>by scintillation counting. If anyone has saved this method
>or has a reference, please could you email or post.
If you want to try using [3H] acetyl CoA, which is cheaper, try the
scintilation assay from:
Rouet, P., Raguenez, G., and Salier, J.-P. (1992)
Biotechniques 13:5 pp. 700-701
It's fast an works wonderfully for me!!
More information about the Methods