Water soluble X-gal like substance

Martin Leach leach at mbcrr.harvard.edu
Mon Oct 18 05:14:36 EST 1993


In article <1993Oct18.102513.333 at chmeds.ac.nz>, mkennedy at chmeds.ac.nz
(Martin Kennedy) wrote:

> In article <andres.40.750707453 at calvin.jci.tju.edu>, andres at calvin.jci.tju.edu (Andres Ferber) writes:
> 
> > Does anybody know of a water soluble cromogenic substrate for beta
> > galactosidase?
> > Thanks
> > Andres
> 
> No, but why not just dissolve the X-gal in a small amount of DMF as usual and 
> then add it into the media or whatever;  it seems to remain in solution 
> (witness its effectiveness on X-gal agar plates etc).

He did try it and told me it pptd out and didn't work!!

someone suggested dissolving it in DMSO as this works better, without
ppting.


.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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