CAT assay

Mr R D Williams lsrgd at csv.warwick.ac.uk
Sat Oct 16 10:14:32 EST 1993


In article <29f5onINNdos at emory.mathcs.emory.edu>,
	djdlab at sun.com writes:
>In article AA25153 at cardinal.ncsc.org, mbwhite at CARDINAL.NCSC.ORG (Martyn White) writes:
>>
>>
>>	Some time ago, someone posted a method for a quick and
>>easy CAT assay involving [3H]chloramphenicol.  The acetylated
>>chloramphenicol is separated by solvent extraction and measured
>>by scintillation counting.  If anyone has saved this method
>>or has a reference, please could you email or post.
>>
>>Thank you
>>
>>Martyn White
>>
>If you want to try using [3H] acetyl CoA, which is cheaper, try the
>scintilation assay from:
>      Rouet, P., Raguenez, G., and Salier, J.-P.  (1992)
>         Biotechniques 13:5 pp. 700-701
>
>It's fast an works wonderfully for me!!
>
>--Tracy Sitler
>
>
>
>
In fact, the Neumann et al/Eastman method also uses [3H] acetyl CoA, and a
scintillant (NEN Econoflour) that doubles as the solvent for extraction of
the [3H] chloramphenicol reaction product (so it's a quick and easy one
tube process).


Richard






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Richard Williams     lsrgd at csv.warwick.ac.uk
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