DNA fragments - gel electrophoreses

Klaus.Matthaei at ANU.EDU.AU Klaus.Matthaei at ANU.EDU.AU
Mon Oct 25 18:08:47 EST 1993


>Dear netters,
>
>have a bit of a quandry.
>
>I have to separate 3 DNA fragments; 5.8, 5.7, and 5.6 kb in size. I require
>the 5.7 kb DNA fragment, or can use a mixture containing the 5.7 and 5.6kb
>fragments.
>
>This is at the end of a lengthy selection procedure. I am wondering if I
>could separate these on a 0.4% or lower agarose gel.
>
>Does anyone have any suggestions at getting the 5.7 kb band, by standard
>electrophoreses.??

Try and find a restriction enzyme(s) that cuts in the other two fragments
and not in yours and use standard separation.


>Please feel free to email me.
>
>Thanx in advance.
>
>Martin Leach
>
>-- 
>
>.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
>   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
>   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
> _/  |-/__==/  80 E. Concord St. (L603)
>(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
>               USA                           head.....WIBBLE" -BLACKADDER  


Cheers, Klaus
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Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at anu.edu.au

"Think twice - Do once"
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