demethylation of DNA

Wayne Woods wgwoods at ariel.ucs.unimelb.EDU.AU
Mon Oct 25 01:11:45 EST 1993


Does anyone know of an enzyme that will remove methyl groups from DNA ?
ie. reverse the effects of dam/dcm methylation?
We would like to be able to demethylate plasmids this way, rather
than grow them in a dam/dcm- E.coli strain.

Thanks for your help,
Wayne.

--------------------------------
Wayne Woods
Dept. Microbiology
University of Melbourne
wgwoods at ariel.ucs.unimelb.edu.au



More information about the Methods mailing list