urea/acrylamide

George Yeung IZZY835 at MVS.OAC.UCLA.EDU
Mon Oct 25 04:16:00 EST 1993


HAVE ANYONE ENCOUNTERED THIS PROBLEM BEFORE :
     I HAVE BEEN DOING IN VITRO RUN-OFF TRANSCRIPTION, AND
ANALYSING THE LABELED RNA PRODUCTS ON UREA/ACRYLAMIDE GELS
I FOUND THAT I ALWAYS HAVE SOME RADIOACTIVE MATERIALS COLLECTING
AT THE SURFACE OF THE WELLS, DESPITE LOADING THE SAMPLE IN 7M UREA
AND HEATING IT TO 85 DEGREES BEFORE LOADING.
ANY SUGGESTIONS ON THIS PROBLEM WILL BE GREATLY APPRECIATED.
GEORGE YEUNG



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