urea/acrylamide

Martin Leach leach at mbcrr.harvard.edu
Mon Oct 25 06:23:18 EST 1993


In article <19931025021628IZZY835 at MVS.OAC.UCLA.EDU>,
IZZY835 at MVS.OAC.UCLA.EDU (George Yeung) wrote:

> HAVE ANYONE ENCOUNTERED THIS PROBLEM BEFORE :
>      I HAVE BEEN DOING IN VITRO RUN-OFF TRANSCRIPTION, AND
> ANALYSING THE LABELED RNA PRODUCTS ON UREA/ACRYLAMIDE GELS
> I FOUND THAT I ALWAYS HAVE SOME RADIOACTIVE MATERIALS COLLECTING
> AT THE SURFACE OF THE WELLS, DESPITE LOADING THE SAMPLE IN 7M UREA
> AND HEATING IT TO 85 DEGREES BEFORE LOADING.
> ANY SUGGESTIONS ON THIS PROBLEM WILL BE GREATLY APPRECIATED.
> GEORGE YEUNG

Find your caps lock key, it hurts my eyes reading caps.

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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