DNase 1 Hypersensitivity

[Michael Holloway]

In article <jwilber-251093120334 at jwilberding.chem.nd.edu> jwilber at pop.nd.edu (Julie Wilberding) writes:

>phenol?chloroform extractions, ALWAYS take the aqueous phase and transfer
>this to a new tube being very careful not to take any organic layer.  After
>you have your aqueous flick your tube and watch for bubbles that sink
>rapidly- that is chloroform and should not be there.  If you leave any
>organic in the tube, you run the risk of star acitivity with your enzyme. 

Don't you precip and dry?  If you haven't brought over visible amounts of 
chloroform this should really take care of it.

Do you see this larger band only in certain cell types?  Does it appear
consistantly?  Are there different sizes that appear?

Mike Holloway
mhollowa at ccmail.sunysb.edu