urea/acrylamide gel

Martin Leach leach at mbcrr.harvard.edu
Tue Oct 26 04:35:27 EST 1993


In article <19931026003151IZZY835 at MVS.OAC.UCLA.EDU>,
IZZY835 at MVS.OAC.UCLA.EDU (George Yeung) wrote:

> Does anyone having the following problem :
> 
>     I have been doing in vitro run-off transcription, and analysed
> the labeled transcripts using urea/acrylamide gels. However, I keep
> finding radioactive materials collecting at the surface of the wells,
> despite loading the samplesin 7M urea and heating the samples to
> 85 degree C before loading.
>     I would really appreciate any suggestions on getting rid of
> this aggregate at the well.

Much better with caps lock off.
Well now, could be some protein left over from the nuclear extract- did you
phenol extract etc..?

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
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(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
               USA                           head.....WIBBLE" -BLACKADDER  



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