urea/acrylamide gel

George Yeung IZZY835 at MVS.OAC.UCLA.EDU
Tue Oct 26 02:31:00 EST 1993


Does anyone having the following problem :

    I have been doing in vitro run-off transcription, and analysed
the labeled transcripts using urea/acrylamide gels. However, I keep
finding radioactive materials collecting at the surface of the wells,
despite loading the samplesin 7M urea and heating the samples to
85 degree C before loading.
    I would really appreciate any suggestions on getting rid of
this aggregate at the well.

George Yeung
izzy835 at mvs.oac.ucla.edu



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