DNA stability

James Herrick jbh2 at cornell.edu
Wed Oct 27 19:20:44 EST 1993


Subject: DNA stability
From: James M Wahlberg, wahlberg at csd4.csd.uwm.edu
Date: 23 Oct 1993 05:01:50 GMT
In article <2aadruINNl9 at uwm.edu> James M Wahlberg,
wahlberg at csd4.csd.uwm.edu writes:
>	Well, you see, I have this friend...  No really.  Someone in our lab
>left some Triton/boiling minipreps (ala The Big Red Book) in isopropyl
>alcohol for >2 weeks.  I guess I'll find out when I get the chance to run
>these preps on a gel whether or not they're any good.  In the mean time,
I
>was wondering if anybody out there has any feel for how stable plasmid
DNA,
>or DNA in general, is upon extended storage in isopropyl alcohol.  
>
>
>			Molecular in Milwaukee

My experience is that DNA is stable almost indefinitely in alcohol. I
believe Sambrook et al. mentions this also (though I may be
misattributing the reference). I lay 10:1 odds your DNA will be just fine.

James B. Herrick
JBH2 at Cornell.edu



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