Proline cis/trans isomerisation

Ben Davis bjd12 at
Thu Oct 28 03:25:10 EST 1993

In article <1993Oct27.135711.17403 at>, smb18 at (Simon Brocklehurst) writes:
|> bjd12 at (Ben Davis) writes:
|>	<stuff deleleted>

|> >	(b) how far (typically) does the cis:trans isomerisation affect the
|> >chemical shifts observed in other residues
|> >	
|>       Which nuclei do you mean???
	1H, 15N. I see what appear to be changes of around 0.25ppm for Ca protons
between two forms, for residues (trp, lys) close to different Pro; this seems
large to me.

|>    As an aside, could I ask how many, if any, disulphides your peptide has?
	None; its a 40mer cleaved from a larger protein (barley CI2) with CNBr	
|> !-----------------------------------------------------------------------
|> Simon M. Brocklehurst
|> Cambridge Centre for Molecular Recognition
|> Department of Biochemistry
|> University of Cambridge
|> Cambridge
|> UK
|> E-mail: smb18 at

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