KM of Taq DNA Polymerase

Paul Riggs riggs at neb.com
Thu Oct 28 12:47:11 EST 1993


In article <01H49DV5N81C8ZE78A at ccvax.sinica.edu.tw>,
BMTYCHANG at CCVAX.SINICA.EDU.TW wrote:
> 
> Hello,
> I would like to know the KM (Michaelis constant) of Taq DNA polymerase from
> Thermus aquaticus (Strain YT1).

Hi Chang
The Km of Taq polymerase for DNA is about 1.4 nM, for triphosphates about
16 uM - see Kong et al.(1993) JBC 268(3)1965-75.

______
Paul Riggs
New England Biolabs
riggs at neb.com



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