RIPA buffer

Helen McBride helen_mcbride at hlthsci.med.utah.edu
Fri Oct 29 18:10:36 EST 1993


	We don't use RIPA buffer here in our lab, but I have used it previously.
The reference for the buffer is in PNAS 87:9396,1990.
Here is my recipe:
20 mm Tris pH 7.4
50mm NaCl
50mm NaF
5mm EDTA
20mm Na pyrophosphate
1mm PMSF
1mm Na orthovanadate
1.0% TritonX-100

In my limited experience the Triton is optional in some usages when lysis
of cells is not needed. The reference should be clearer on this point.
Hope this helps.
Helen_McBride at hlthsci.med.utah.edu



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