E.coli expression sysytems
hersh at macc.wisc.edu
Fri Oct 29 15:33:42 EST 1993
In article <1993Oct29.150600.1 at molbiol.ox.ac.uk>, iwilson at molbiol.ox.ac.uk
> I have asked the student in our group for fuller details: she writes:
> "In my experience a GST-fusion of EPO was better than His6-EPO....
> Talking to others at the Inst. Mol. Med. in Oxford,...
> So to summarise, I think it's very difficult to generalise. The only solution
> is to try the systems and see which is best for the particular protein.
> Iain Wilson
> Dyson Perrins Laboratory, University of Oxford
Thanks for such a thorough response! Alas, like many things in protein
purification, it's seems to be quite a hit-or-miss affair. Good Luck.
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