E.coli expression sysytems

Rick Hershberger hersh at macc.wisc.edu
Fri Oct 29 15:33:42 EST 1993


In article <1993Oct29.150600.1 at molbiol.ox.ac.uk>, iwilson at molbiol.ox.ac.uk
wrote:

> I have asked the student in our group for fuller details: she writes:
> 
> "In my experience a GST-fusion of EPO was better than His6-EPO....

  [paragraph deleted]

> Talking to others at the Inst. Mol. Med. in Oxford,...

  [paragraph deleted]

> So to summarise, I think it's very difficult to generalise. The only solution 
> is to try the systems and see which is best for the particular protein.

  [paragraph deleted]
 
> Iain Wilson
> Dyson Perrins Laboratory, University of Oxford

Thanks for such a thorough response!  Alas, like many things in protein
purification, it's seems to be quite a hit-or-miss affair.  Good Luck.

Rick Hershberger



More information about the Methods mailing list