Concentrating small, dilute DNA

swiatek at mbcl.rutgers.edu swiatek at mbcl.rutgers.edu
Fri Oct 22 19:20:14 EST 1993


Greetings,
	Does anyone know of the best way to concentrate (i.e. ppt) small
	DNAs (<300bp)? I'm using Promega's Magic PCR preps, and the DNA is
	eluted in 40 to 50ul of buffer (or water, in my case). Currently,
	I have about 2ug DNA in 80ul H2O and need to concentrate it for 
	ligation. Which salts work best? Also, is isopropanol or EtOH better?
	
	Thanks for any and all help...
---------------------------------------------------------------------------
Gavin Swiatek                                   /
Dept Micro & Molec Genetics                     /        E
Rutgers Univ                                    /    Pluribus
New Brunswick, NJ                               /   Pandemonium.....
---------------------------------------------------------------------------
  



More information about the Methods mailing list