Concentrating small, dilute DNA
swiatek at mbcl.rutgers.edu
swiatek at mbcl.rutgers.edu
Fri Oct 22 19:20:14 EST 1993
Greetings,
Does anyone know of the best way to concentrate (i.e. ppt) small
DNAs (<300bp)? I'm using Promega's Magic PCR preps, and the DNA is
eluted in 40 to 50ul of buffer (or water, in my case). Currently,
I have about 2ug DNA in 80ul H2O and need to concentrate it for
ligation. Which salts work best? Also, is isopropanol or EtOH better?
Thanks for any and all help...
---------------------------------------------------------------------------
Gavin Swiatek /
Dept Micro & Molec Genetics / E
Rutgers Univ / Pluribus
New Brunswick, NJ / Pandemonium.....
---------------------------------------------------------------------------
More information about the Methods
mailing list